Enzyme structure, catalysis and environmental effects on enzyme function

Learning objectives

Explain how an enzyme lowers activation energy without being consumed, and describe the induced-fit interaction between the active site and its substrate

Explain how enzyme specificity follows from the shape and chemistry of the active site, and why a change in the primary sequence of the enzyme may change or destroy its activity

Predict and explain the effect of temperature, pH, substrate concentration and enzyme concentration on reaction rate, including saturation and denaturation

Distinguish competitive from non-competitive inhibition by the site of binding and by the effect on the rate at high substrate concentration, and explain allosteric regulation and feedback inhibition

Design a controlled investigation of one factor affecting enzyme activity, and use the data to justify a claim about the enzyme's optimum conditions